On-off controllable RNA hybrid expression vector for yeast three-hybrid system

On-off controllable RNA hybrid expression vector for yeast three-hybrid system.

The yeast three-hybrid system (Y3H), a powerful method for identifying RNA-binding proteins, still suffers from many false positives, due mostly to RNA-independent interactions. In this study, we attempted to efficiently identify false positives by introducing a tetracycline operator (tetO) motif into the RPR1 promoter of an RNA hybrid expression vector. We successfully developed a tight tetrac...

Stable DNA-binding yeast vector allowing high-bait expression for use in the two-hybrid system.

Designing a hybrid quantum controller for strongly eigenstate controllable systems

 In this paper, a new quantum hybrid controller for controlling the strongly eigenstate controllable systems, is designed. For this purpose, a Lyapunov control law is implemented when the target state is in reachable set of the initial state. On the other hand, if the target state is not in the reachable set of the given initial state, based on Grover algorithm, a new interface state that the t...

Yeast two-hybrid system

Introduction The two-hybrid system (THS), first introduced by Fields and Song (1989) (Ref. 1), is a powerful technique for identifying new proteins involved in specific biological processes. It allows for the rapid isolation of the gene that codes for a protein that interacts with a specific protein of interest. Here, we present not a review of the current THS technology but, rather, a comprehe...

A yeast RNA-hybrid system for the detection of RNA-RNA interactions in vivo.

RNA-RNA interactions play a crucial role at many different levels of the cellular metabolism such as plasmid replication control, viral encapsidation, or transcriptional and translational regulation. Therefore, methods are necessary to investigate the molecular determinants of given interactions, including their stabilities, or to screen for new interacting partners. We designed an RNA-hybrid s...